Publications
Cell Biology
The articles listed below all contain images and/or references to the BZ Series Fluorescence Microscope and is by no means exhaustive. Please click on the links below to view the article in its entirety. For additional information on the BZ Fluorescence Microscope or other research areas that make use of our fluorescence microscope, please contact your local KEYENCE representative using our support services on the right.
The Atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice
[HTML] from molbiolcell.org Y Sou, S Waguri, J Iwata, T Ueno… - … Biology of the Cell, 2008 - Am Soc Cell Biol ... Fluorescence images were obtained using a fluorescence microscope (model BZ-9000; Keyence, Osaka, Japan). Atg16L-positive dots per each treated cell (n = 20) were counted using software Dynamic cell count BZ-HIC (Keyence). ...
Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells
[PDF] from sussex.ac.uk T Ogi, S Limsirichaikul, RM Overmeer, M Volker… - Molecular cell, 2010 - Elsevier
An EF‐hands protein, centrin‐1, is an EGTA‐sensitive SUMO‐interacting protein in mouse testis
[PDF] from sumo-modification.jp N Tanaka, M Goto, A Kawasaki… - Cell Biochemistry …, 2010 - Wiley Online Library ... bacterial lysate or purified EGFP–SUMO-3 in the presence or absence of EGTA/CaCl 2 . As soon as the incubation started, the beads were viewed in real-time under equilibrium conditions and photographed using a BIOREVO BZ-9000 fluorescence microscope (Keyence). ...
Molecular and biophysical properties of voltage-gated Na+ channels in murine vas deferens
[HTML] from nih.gov HL Zhu, M Aishima, H Morinaga, RD Wassall… - Biophysical journal, 2008 - Elsevier ... Coverslips were mounted onto slides by use of fluorescence mounting medium and slides were viewed by fluorescent microscopy (Biozero BZ-8000, Keyence). Mouse cerebrum was used as a positive control for anti-Na V 1.1 and anti-Na V 1.2 antibodies. Na V 1.6-null mice. ...
Autophagic elimination of misfolded procollagen aggregates in the endoplasmic reticulum as a means of cell protection
[HTML] from molbiolcell.org Y Ishida, A Yamamoto, A Kitamura… - … Biology of the Cell, 2009 - Am Soc Cell Biol ... Cell counting was performed using a Biozero digital microscope (Keyence, Osaka, Japan) equipped with a PlanFluor 20x, 0.50 NA objective (Nikon, Tokyo, Japan)...
Autophagic elimination of misfolded procollagen aggregates in the endoplasmic reticulum as a means of cell protection
[HTML] from molbiolcell.org Y Ishida, A Yamamoto, A Kitamura… - … Biology of the Cell, 2009 - Am Soc Cell Biol ... Cell counting was performed using a Biozero digital microscope (Keyence, Osaka, Japan) equipped with a PlanFluor 20x, 0.50 NA objective (Nikon, Tokyo, Japan)...
The Short Arm of Laminin {gamma} 2 Chain of Laminin-5 (Laminin-332) Binds Syndecan-1 and Regulates Cellular Adhesion and Migration by Suppressing …
[HTML] from molbiolcell.org T Ogawa, Y Tsubota, J Hashimoto… - … biology of the cell, 2007 - Am Soc Cell Biol ... Laboratories, Burlingame, CA) was used for detection. Fluorecsence images were obtained using a fluorescence microscope (model BZ-8000; Keyence, Osaka, Japan). SDS-Polyacrylamide Gel Electrophoresis (PAGE) and ...
Quinolinate phosphoribosyl transferase, a key enzyme in de novo NAD+ synthesis, suppresses spontaneous cell death by inhibiting overproduction of active-caspase- …
K Ishidoh, N Kamemura, T Imagawa, M Oda… - … (BBA)-Molecular Cell …, 2010 - Elsevier ... The samples were mounted on slide glasses using Aqua/Polymount (Polysciences, Warrington, PA) and observed on a Leica TSC NT confocal microscope (Leica Instruments, Nusslosh, Germany) or fluorescence microscope (model BIOREVO BZ-9000, Keyence, Osaka). 2.6. ...
Competitive nuclear export of cyclin D1 and Hic-5 regulates anchorage dependence of cell growth and survival
[HTML] from molbiolcell.org K Mori, E Hirao, Y Toya, Y Oshima… - … Biology of the Cell, 2009 - Am Soc Cell Biol ... Fluorescence microscopy was performed using a RTS-2000 MP confocal laser microscope (Bio-Rad Laboratories, Hercules, CA) and a BZ-8000 microscope (Keyence, Osaka, Japan), and the data were analyzed using BZ-Analyzer software (Keyence). ...
A critical importance of polyamine site in NMDA receptors for neurite outgrowth and fasciculation at early stages of P19 neuronal differentiation
D Georgiev, H Taniura, Y Kambe, T Takarada… - Experimental cell …, 2008 - Elsevier ... Iwaki, Japan). Phase contrast and fluorescence images were obtained using Fluorescence Microscope (BZ-8000; Keyence, Osaka, Japan) using BZ Analyzer Software (Keyence). Assessment of cell viability. P19 neuronal cultures ...
POU6F1 is the transcription factor that might be involved in cell proliferation of clear cell adenocarcinoma of the ovary
N YOSHIOKA, N SUZUKI, A UEKAWA… - … Cell, 2009 - Wiley Online Library ... rinsing three times with PBS, two drops of Vecta Shield were added and the cells were covered with a slide glass and let stand at room temperature in the dark for 1 h. Then the results of immunostaining were evaluated by fluorescence microscope (BZ-9000; Keyence, Osaka ...
High tolerance to apoptotic stimuli induced by serum depletion and ceramide in side-population cells: high expression of CD55 as a novel character for side- …
JX Xu, E Morii, Y Liu, N Nakamichi, J Ikeda… - Experimental cell …, 2007 - Elsevier ... The annexin V + cells were visualized with a fluorescent microscope (BZ-8000, Keyence, Osaka, Japan), and the proportion of annexin V + cells to total cells was
counted. At least 5 × 10 2 cells were counted for each experiment. ...
Adiponectin stimulates AMP-activated protein kinase in the hypothalamus and increases food intake
N Kubota, W Yano, T Kubota, T Yamauchi, S Itoh… - Cell metabolism, 2007 - Elsevier
A comparison of the tube forming potentials of early and late endothelial progenitor cells
N Mukai, T Akahori, M Komaki, Q Li… - Experimental cell …, 2008 - Elsevier ... These cells were mixed with HUVECs which were pre-labeled with PKH26 Red, and cultured on the substrate. The fluorescence of EPC and HUVECs was analyzed by a fluorescence microscopy (BZ-8000; Keyence, Osaka, Japan). ...
Laminin [alpha] 5 mediates ectopic adhesion of hepatocellular carcinoma through integrins and/or Lutheran/basal cell adhesion molecule
Y Kikkawa, R Sudo, J Kon, T Mizuguchi… - Experimental cell …, 2008 - Elsevier ... Images captured using a Zeiss LSM510 confocal microscope (Carl Zeiss, Oberkochen, Germany) and Biozero (Keyence, Osaka, Japan) were imported into Adobe Photoshop CS and Adobe Illustrator CS (San Jose, CA) for processing and layout. Table 1. Primary antibodies ...
Drought stress alters water relations and expression of PIP-type aquaporin genes in Nicotiana tabacum plants
M Mahdieh, A Mostajeran, T Horie… - Plant and Cell …, 2008 - Jpn Soc Plant Physiol ... After 1–2 d, GFP fluorescence of individual oocytes was analyzed using a BioZero laser scanning microscope (objective lens ×20, BZ-8000, KEYENCE Corporation, Osaka, Japan) with 470 nm excitation and 535 nm emission filters (company information). ...
Rapid decrease in active tension generated by C2C12 myotubes after termination of artificial exercise
H Fujita, M Hirano, K Shimizu… - … of muscle research and cell …, 2010 - Springer ... The specimens were washed three times with PBS and then studied under a fluorescence microscope (BZ-9000; Keyence, Tokyo, Japan). Live cell imaging ... Then, cells were observed under a fluorescence microscope (BZ-9000). ...
Distinct roles of synaptic transmission in direct and indirect striatal pathways to reward and aversive behavior
T Hikida, K Kimura, N Wada, K Funabiki… - Neuron, 2010 - Elsevier
Beneficial storage effects of epigallocatechin-3-o-gallate on the articular cartilage of rabbit osteochondral allografts
JY Bae, K Matsumura, S Wakitani… - Cell …, 2009 - ingentaconnect.com ... The tissue blocks were cut into 5-μm sections and stained with either Safranin-O/fast green or toluidine blue for evaluating GAG content and distribution. The prepared sections were examined using an optical/fluorescence microscope (Biozero-8000, Keyence, Osaka, Japan). ...
Long-term preservation of rat skin tissue by epigallocatechin-3-o-gallate
HH Kim, T Kawazoe, K Matsumura… - Cell …, 2009 - ingentaconnect.com ... Ltd. Tokyo, Japan) for cryosectioning and further GFP fluorescent confirmation. The prepared sections were all observed under an optical/fluorescence microscope (Biozero-8000, Keyence, Osaka, Japan). Statistical Analysis ...
Cytosolic chaperonin prevents polyglutamine toxicity with altering the aggregation state
[PDF] from northwestern.edu A Kitamura, H Kubota, CG Pack, G Matsumoto… - Nature cell …, 2006 - nature.com ... collagen for 16 h. Immediately after transfection of Htt–GFP expression vectors, neuronal cell differentiation was induced by addition of 5 mM dibutyryl cAMP for 72 h. Cells stained with propidium iodide (1 g ml -1 ) were counted as dead cells using a Biozero digital microscope ...
Rev-derived peptides inhibit HIV-1 replication by antagonism of Rev and a co-receptor, CXCR4
K Shimane, EN Kodama, I Nakase, S Futaki… - … of Biochemistry & Cell …, 2010 - Elsevier ... After 48 h, the cells were fixed with 1% formaldehyde and examined with a BioZERO fluorescence microscope (BZ-8000; Keyence, Osaka, Japan). 3. Results. 3.1. Arginine-rich peptides inhibit HIV-1 IIIB infection. The amino acid sequences of the peptides are shown in Fig. ...
Biological and Biomechanical Evaluations of Osteochondral Allografts Preserved in Cold Storage Solution Containing Epigallocatechin Gallate
JY Bae, DW Han, S Wakitani… - Cell Transplantation …, 2010 - ingentaconnect.com ... antibody (Ab) against type II collagen. The prepared sections were examined using an optical/fluorescence microscope (Biozero-8000, Keyence, Osaka, Japan). Biomechanical Analysis At each data point, cartilage specimens ...
Adiponectin Enhances Insulin Sensitivity by Increasing Hepatic IRS-2 Expression via a Macrophage-Derived IL-6-Dependent Pathway
M Awazawa, K Ueki, K Inabe, T Yamauchi… - Cell Metabolism, 2011 - Elsevier
p53 regulates glucose metabolism through an IKK-NF-κB pathway and inhibits cell transformation
[PDF] from korea.ac.kr K Kawauchi, K Araki, K Tobiume… - nature cell biology, 2008 - nature.com ... supplemented with 600 M 2-NBDG (2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2- deoxyglucose; Molecular Probes) and 3.3 mM glucose for 10 min at 37°C. After washing with KRB buffer, MEFs were imaged at 488 nm using a fluorescence microscope (Keyence BZ-8000). ...
Distribution of prosaposin in the rat nervous system
Y Hosoda, K Miyawaki, S Saito, J Chen… - Cell and Tissue …, 2007 - Springer ... The sections were rinsed with PBS, stained with 4'-6'-diamidino-2-phenylindole (DAPI), rinsed twice with PBS, mounted in Mowiol (Calbiochem, San Diego, CA, USA), and examined under a fluorescence microscope (BZ-8000; Keyence, Osaka, Japan). ...
Apaf-1-independent programmed cell death in mouse development
A Nagasaka, K Kawane, H Yoshida… - Cell Death & …, 2009 - nature.com ... Sections were mounted with Mount-Quick (Daido Sangyo, Toda, Saitama, Japan) or Fluoromount (Diagnostic BioSystems, Pleasanton, CA, USA), and observed by fluoroescence microscopy (IX-70; Olympus, Tokyo, Japan or BioRevo BZ-9000; Keyence, Osaka, Japan). ...
Involvement of extracellular oxidative burst in salicylic acid‐induced stomatal closure in Arabidopsis
MD KHOKON, E OKUMA, MA HOSSAIN… - Plant, Cell & … - Wiley Online Library ... The epidermal peels were incubated for 40 min at room temperature, and then the excess dye was washed out with distilled water.The dye-loaded tissues were treated with 500 mM SA, and then fluorescence of guard cells were imaged using Bio-zero software. ...
Therapeutic angiogenesis by transplantation of induced pluripotent stem cell-derived Flk-1 positive cells
[PDF] from biomedcentral.com H Suzuki, R Shibata, T Kito, M Ishii, P Li… - BMC Cell …, 2010 - biomedcentral.com ... incubated at 37 °C for 24 h. Network formation and Flk-1 + iPS cell incorporation were assessed using an inverted phase contrast microscope (Biozero BZ8000, KEYENCE Japan, Osaka, Japan). Mouse model of hind limb ischemia and cell transplantation ...
Effects of human full-length amelogenin on the proliferation of human mesenchymal stem cells derived from bone marrow
YC Huang, K Tanimoto, Y Tanne, T Kamiya… - Cell and Tissue Research - Springer ... Ready-to-use VECTASTAIN Universal Quick Kit (Vector Laboratories, Burlingame, CA, USA) was used for staining. The distribution of LAMP1 was observed microscopically (BZ-8100; Keyence, Osaka, Japan). Analysis of MAPK-ERK activity ...
Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression
K Kuwako, K Kakumoto, T Imai, M Igarashi… - Neuron, 2010 - Elsevier
Regulation of the susceptibility to oxidative stress by cysteine availability in pancreatic β-cells
[HTML] from physiology.org
S Numazawa, H Sakaguchi, R Aoki… - … of Physiology-Cell …, 2008 - Am Physiological Soc ... Nuclei were stained with 4',6-diamino-2-phenylindole (DAPI). Subcellular localization of the PDX-1 was observed using an inverted fluorescent microscopy (Keyence BZ-8000). Statistical analysis. All data are given as the means ± SD.
Function of GRAS Proteins in Root Nodule Symbiosis is Retained in Homologs of a Non-Legume, Rice
K Yokota, T Soyano, H Kouchi… - Plant and cell …, 2010 - Jpn Soc Plant Physiol ... SZX12, Olympus). For observation of infection threads, samples were analyzed under an epifluorescence microscope (BZ-9000, Keyence) using a filter set (excitation BP560–600, dichroic 595, emission BP630–690). For light ...
Rice NON-YELLOW COLORING1 is involved in light-harvesting complex II and grana degradation during leaf senescence
[HTML] from nih.gov M Kusaba, H Ito, R Morita, S Iida… - The Plant Cell …, 2007 - Am Soc Plant Biol
Clonally amplified cardiac stem cells are regulated by Sca-1 signaling for efficient cardiovascular regeneration
[HTML] from biologists.org K Tateishi, E Ashihara, N Takehara… - Journal of Cell …, 2007 - jcs.biologists.org ... an ApopTag kit (Chemicon). H 2 O 2 was purchased from Wako. Images were captured with a BZ-8000 (Keyence, Japan) and IX 71 (Olympus Corporation, Japan). Myocardial infarction and cell grafting. Ligation of the left anterior ...
Differentiation of induced pluripotent stem cells to thymic epithelial cells by phenotype
Y Inami, T Yoshikai, S Ito, N Nishio… - Immunology and Cell …, 2010 - nature.com ... for 5 min. After dehydration, cells were mounted with mounting medium (Malinol), observed and photographed using OLYMPUS BX50F (Tokyo, Japan), or a Keyence BZ-8000 microscope (Osaka, Japan). For immunostaining ...
Cholesterol homeostasis in ABCA1/LCAT double-deficient mouse
MA Hossain, M Tsujita, N Akita, F Kobayashi… - … BBA)-Molecular and Cell …, 2009 - Elsevier ... The frozen sections were washed three times with PBS and stained with Oil red O. Tissues were observed in a Biozero microscope system BZ8000 (Keyence Japan). 2.5. Western blotting analysis of protein. ABCA1 protein in the liver was analyzed by Western blotting [20]. ...
miR-22 represses cancer progression by inducing cellular senescence
D Xu, F Takeshita, Y Hino, S Fukunaga… - The Journal of Cell …, 2011 - jcb.rupress.or
NPR-A regulates self-renewal and pluripotency of embryonic stem cells
EM Abdelalim… - Cell Death & Disease, 2011 - nature.com ... The slides were examined by fluorescence microscopy (Biozero; Keyence, Osaka, Japan) or by confocal laser microscopy (C1si; Nikon, Tokyo, Japan), and images were processed using Nikon EZ-C1 viewer software. RT-PCR and real-time PCR. ...
Difference in the distribution and speciation of cellular nickel between nickel‐tolerant and non‐tolerant Nicotiana tabacum L. cv. BY‐2 cells
A SAITO, M SAITO, Y ICHIKAWA… - Plant, Cell & …, 2010 - Wiley Online Library ... Protoplast viability was determined as a percentage of the value obtained in the control experiment (n = 50–110 cells) (c). The number of living cells that could produce fluorescence was counted by an all-in-one type fluorescent microscope (BZ-8000; Keyence, Tokyo, Japan). ...
Stress-induced non-vesicular release of prothymosin-α initiated by an interaction with S100A13, and its blockade by caspase-3 cleavage
H Matsunaga… - Cell Death & Differentiation, 2010 - nature.com ... Images were collected using a BZ-8000 microscope (KEYENCE, Osaka, Japan) with a × 20 Plan APO lens (Nikon, Tokyo, Japan) or using an LSM 510 META confocal laser microscope with a × 40 Plan-Neofluar lens or a × 63 Plan-Apochromat lens (Carl Zeiss, Oberkochen ...
Mechanisms of Water Transport Mediated by PIP Aquaporins and Their Regulation Via Phosphorylation Events Under Salinity Stress in Barley Roots
T Horie, T Kaneko, G Sugimoto… - Plant and Cell …, 2011 - Jpn Soc Plant Physiol ... IgG goat antibody conjugated with Alexa 647 and anti-rabbit IgG goat antibody conjugated with Alexa 488 (Invitrogen)] for another 1 h. After washing twice with the blocking solution and once with PBS, samples were analyzed with a fluorescence microscope (BZ-8000, Keyence ...
Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells
Z Cheng, S Ito, N Nishio, H Xiao… - … of Molecular Cell …, 2011 - jmcb.oxfordjournals.org ... Cells were incubated with optimal dilutions of primary antibody and followed by fluorescence- labeled appropriate antibody. They were observed and photographed with a Keyence BZ-8000 microscope. Detection of iPS cells by marker staining. ES-AP detection kit (Cat no. ...
